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| Veiga,G.A.L.; Milazzotto,M.P.; Nichi,M.; Lúcio,C.F.; Silva,L.C.G.; Angrimani,D.S.R.; Vannucchi,C.I.. |
| In the canine species, the precise mechanisms of pregnancy maintenance and the initiation of parturition are not completely understood. The expression of genes encoding the receptors for estrogen (ERα mRNA) and oxytocin (OTR mRNA) was studied in the endometrium and myometrium during pregnancy and parturition in dogs. Real-time PCR was performed to quantify the levels of ERα mRNA and OTR mRNA in the uterus of bitches during early (up to 20 days of gestation), mid (20 to 40 days) and late pregnancy (41 to 60 days), and parturition (first stage of labor). All tissues expressed ERα and OTR mRNA, and are thus possibly able to respond to eventual estrogen and oxytocin hormonal stimuli. No statistically significant differences in the expression of ERα mRNA were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine; Estrogen; Oxytocin; Receptors; Pregnancy; Parturition. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000400339 |
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| Mello,M.R.B.; Caetano,H.V.A.; Marques,M.G.; Padilha,M.S.; Garcia,J.F.; Milazzotto,M.P.; Assumpção,M.E.O.A.; Lima,A.S.; Nicácio,A.C.; Mendes,C.M.; Oliveira,V.P.; Visintin,J.A.. |
| The present study examined the in vitro and in vivo development of bovine nuclear-transferred embryos. A bovine fetal fibroblast culture was established and used as nucleus donor. Slaughterhouse oocytes were matured in vitro for 18 h before enucleation. Enucleated oocytes were fused with fetal fibroblasts with an electric stimulus and treated with cytochalasin D and cycloheximide for 1 h followed by cycloheximide alone for 4 h. Reconstructed embryos were cultured for 7-9 days and those which developed to blastocysts were transferred to recipient cows. Of 191 enucleated oocytes, 83 (43.5%) were successfully fused and 24 (28.9%) developed to blastocysts. Eighteen freshly cloned blastocysts were transferred to 14 recipients, 5 (27.8%) of which were pregnant... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cloning; Nuclear transfer; Bovine; Fetal fibroblasts; Embryo reconstruction. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100006 |
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